For decades, optimizing annealing temperatures in PCR has pushed the limits of PCR forward to more difficult tasks. At the same time, mastermixes and kits became more powerful and less prone for ...

Failed polymerase chain reactions (PCRs) are a common cause of frustration in the lab. While a typical PCR protocol is quite straightforward, scientists must optimize each step to amplify the intended ...

PCR involves three core components: polymerase, primer, and template. Primers anneal to templates and create initiation sites for the polymerase. The polymerase then recruits free deoxynucleoside ...

PCR products resulting from the gradient runs for determination of (A) annealing and (B) denaturation temperatures, respectively. The appropriate gradient temperatures [°C] of both runs are inserted ...

Polymerase chain reaction (PCR) is a technique that has revolutionized the world of molecular biology and beyond, enabling the amplification of nucleotide sequences. In this article, we will discuss a ...

In this video from Thermo Fisher Scientific, a few tips for better PCR primer design are presented. PCR, Polymerase Chain Reaction, is a common and vital part of genetic studies, biomedical research ...

In 1983, American biochemist Kary Mullis was driving home late at night when a flash of inspiration struck him. He wrote on the back of a receipt the idea that would eventually grant him the Nobel ...