Nature

A capless hairpin-protected mRNA vaccine encoding the full-length Influenza A hemagglutinin protects mice against a lethal Influenza A infection

The success of mRNA vaccines in controlling the COVID 19 pandemic has confirmed the efficacy of synthetically synthesized mRNA in humans and has also provided a blueprint on how to design them in ...
Nature

Circular Vectors as an efficient, fully synthetic, cell-free approach for preparing small circular DNA as a plasmid substitute for guide RNA expression in CRISPR–Cas9 genome ...

Robust expression of guide RNA (gRNA) is essential for successful implementation of CRISPR–Cas9 genome-editing methods. The gRNA components, such as an RNA polymerase promoter followed by the gRNA ...
GEN

Gene Syntax Guidelines to Increase Expression and Predictability

Sizeable differences in gene expression means and ratios—based solely upon where genes are located on a plasmid (gene syntax)—have been discovered by researchers at Dartmouth College. This new ...
Nanowerk

Plasmid: Structure, Function, and Role in Biotechnology and Medicine

The term “plasmid” was coined by Joshua Lederberg in 1952 to describe extrachromosomal genetic elements observed during studies of bacterial conjugation and antibiotic resistance. Their transformation ...
Phys.org

A new approach to controlling gene expression in microbial populations

The ability to control gene expression across a wide range is crucial for many fields, including bioengineering, disease detection, and environmental monitoring. Research led by Lingchong You, Ph.D., ...