The GoTaq ® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq ® ...
Ten PCR assays targeting five biological agents (Bacillus anthracis, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis, and Yersinia pestis) were incorporated onto Array Cards. A ...
Premier Biosoft’s AlleleID Was Designed to Assist in Development of Diagnostic Assays Efficient clinical diagnosis of pathogens is important for the management of infectious diseases. Conventional ...
Expert Rev Mol Diagn. 2012;12(7):731-754. SYBR Green and dual-hybridization probes are often used for melting point analysis at the end of the amplification. qPCR presents a number of advantages over ...
Polymerase Chain Reaction (PCR) is a method which utilizes the mechanism of DNA replication to amplify a specific part of DNA. This involves the use of DNA polymerase, which makes new strands of DNA ...
To assess COLD-PCR enrichment, many different assays can be applied, either in combination with COLD-PCR (real-time COLD-PCR [35]) or downstream, in order to analyze enriched genetic variations. The ...