qPCR Analysis - Standard Curve Method
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YouTubePrecision Health
qPCR Analysis - Standard Curve Method
To generate the standard curve, we first need to prepare four 3-fold serials dilutions of cDNA template known to express the gene of interest in high abundance, as a calibrator sample. If you do not have a cDNA template that has high expression of the gene of interest, you can generate the calibrator by pooling the cDNA from your unknown sample ...
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